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neuronal labelling neurobiotin  (Vector Laboratories)


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    Vector Laboratories neuronal labelling neurobiotin
    OB neurones were distinguished by their anatomical and electrophysiological properties in blind whole-cell recordings. Ai and Bi, reconstructions of <t>neurobiotin-labelled</t> cells from horizontal OB sections (300 μm). GC interneurones (Ai) were characterized by widely branching dendrites projecting rostrally in horizontal sections. GC dendrites were covered in gemmules and long-necked spines, up to 10 μm in length (insets - photomicrographs of the regions delineated by the boxes; arrowheads mark cell somata). MTC dendrites, by contrast, were aspiny. In the MTC shown (Bi) an apical dendrite can be followed rostrally, where it ramifies in an olfactory glomerulus (inset). Aii and Bii, traces of spontaneous synaptic activity from the cells in Ai and Bi show the characteristic differences between GC and MTC electrophysiology at rest. In the nose-brain preparation, large (> 5 mV) spontaneous depolarizing potentials in GCs occurred at rates of ≈5 Hz, while spontaneous synaptic activity was less prominent in MTCs. Rinput, input resistance. Aiii and Biii, lateral olfactory tract (LOT) stimulation evoked an antidromic AP in MTCs, which, in turn, synaptically activated the GC population. The antidromic AP in MTCs rose straight from baseline. Hyperpolarization (lower trace) did not block the AP but reversed the synaptically mediated after-hyperpolarization (the AP is truncated in the lower trace). In GCs, LOT stimulation produced an EPSP that increased at more hyperpolarized levels, while the AP was blocked.
    Neuronal Labelling Neurobiotin, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 95/100, based on 98 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/neuronal labelling neurobiotin/product/Vector Laboratories
    Average 95 stars, based on 98 article reviews
    neuronal labelling neurobiotin - by Bioz Stars, 2026-03
    95/100 stars

    Images

    1) Product Images from "Contribution of a calcium-activated non-specific conductance to NMDA receptor-mediated synaptic potentials in granule cells of the frog olfactory bulb"

    Article Title: Contribution of a calcium-activated non-specific conductance to NMDA receptor-mediated synaptic potentials in granule cells of the frog olfactory bulb

    Journal:

    doi: 10.1113/jphysiol.2002.024638

    OB neurones were distinguished by their anatomical and electrophysiological properties in blind whole-cell recordings. Ai and Bi, reconstructions of neurobiotin-labelled cells from horizontal OB sections (300 μm). GC interneurones (Ai) were characterized by widely branching dendrites projecting rostrally in horizontal sections. GC dendrites were covered in gemmules and long-necked spines, up to 10 μm in length (insets - photomicrographs of the regions delineated by the boxes; arrowheads mark cell somata). MTC dendrites, by contrast, were aspiny. In the MTC shown (Bi) an apical dendrite can be followed rostrally, where it ramifies in an olfactory glomerulus (inset). Aii and Bii, traces of spontaneous synaptic activity from the cells in Ai and Bi show the characteristic differences between GC and MTC electrophysiology at rest. In the nose-brain preparation, large (> 5 mV) spontaneous depolarizing potentials in GCs occurred at rates of ≈5 Hz, while spontaneous synaptic activity was less prominent in MTCs. Rinput, input resistance. Aiii and Biii, lateral olfactory tract (LOT) stimulation evoked an antidromic AP in MTCs, which, in turn, synaptically activated the GC population. The antidromic AP in MTCs rose straight from baseline. Hyperpolarization (lower trace) did not block the AP but reversed the synaptically mediated after-hyperpolarization (the AP is truncated in the lower trace). In GCs, LOT stimulation produced an EPSP that increased at more hyperpolarized levels, while the AP was blocked.
    Figure Legend Snippet: OB neurones were distinguished by their anatomical and electrophysiological properties in blind whole-cell recordings. Ai and Bi, reconstructions of neurobiotin-labelled cells from horizontal OB sections (300 μm). GC interneurones (Ai) were characterized by widely branching dendrites projecting rostrally in horizontal sections. GC dendrites were covered in gemmules and long-necked spines, up to 10 μm in length (insets - photomicrographs of the regions delineated by the boxes; arrowheads mark cell somata). MTC dendrites, by contrast, were aspiny. In the MTC shown (Bi) an apical dendrite can be followed rostrally, where it ramifies in an olfactory glomerulus (inset). Aii and Bii, traces of spontaneous synaptic activity from the cells in Ai and Bi show the characteristic differences between GC and MTC electrophysiology at rest. In the nose-brain preparation, large (> 5 mV) spontaneous depolarizing potentials in GCs occurred at rates of ≈5 Hz, while spontaneous synaptic activity was less prominent in MTCs. Rinput, input resistance. Aiii and Biii, lateral olfactory tract (LOT) stimulation evoked an antidromic AP in MTCs, which, in turn, synaptically activated the GC population. The antidromic AP in MTCs rose straight from baseline. Hyperpolarization (lower trace) did not block the AP but reversed the synaptically mediated after-hyperpolarization (the AP is truncated in the lower trace). In GCs, LOT stimulation produced an EPSP that increased at more hyperpolarized levels, while the AP was blocked.

    Techniques Used: Activity Assay, Blocking Assay, Produced



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    neuronal labelling neurobiotin  (Vector Laboratories)
    95
    Vector Laboratories neuronal labelling neurobiotin
    OB neurones were distinguished by their anatomical and electrophysiological properties in blind whole-cell recordings. Ai and Bi, reconstructions of <t>neurobiotin-labelled</t> cells from horizontal OB sections (300 μm). GC interneurones (Ai) were characterized by widely branching dendrites projecting rostrally in horizontal sections. GC dendrites were covered in gemmules and long-necked spines, up to 10 μm in length (insets - photomicrographs of the regions delineated by the boxes; arrowheads mark cell somata). MTC dendrites, by contrast, were aspiny. In the MTC shown (Bi) an apical dendrite can be followed rostrally, where it ramifies in an olfactory glomerulus (inset). Aii and Bii, traces of spontaneous synaptic activity from the cells in Ai and Bi show the characteristic differences between GC and MTC electrophysiology at rest. In the nose-brain preparation, large (> 5 mV) spontaneous depolarizing potentials in GCs occurred at rates of ≈5 Hz, while spontaneous synaptic activity was less prominent in MTCs. Rinput, input resistance. Aiii and Biii, lateral olfactory tract (LOT) stimulation evoked an antidromic AP in MTCs, which, in turn, synaptically activated the GC population. The antidromic AP in MTCs rose straight from baseline. Hyperpolarization (lower trace) did not block the AP but reversed the synaptically mediated after-hyperpolarization (the AP is truncated in the lower trace). In GCs, LOT stimulation produced an EPSP that increased at more hyperpolarized levels, while the AP was blocked.
    Neuronal Labelling Neurobiotin, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/neuronal labelling neurobiotin/product/Vector Laboratories
    Average 95 stars, based on 1 article reviews
    neuronal labelling neurobiotin - by Bioz Stars, 2026-03
    95/100 stars
    		  Buy from Supplier

    Image Search Results


    OB neurones were distinguished by their anatomical and electrophysiological properties in blind whole-cell recordings. Ai and Bi, reconstructions of neurobiotin-labelled cells from horizontal OB sections (300 μm). GC interneurones (Ai) were characterized by widely branching dendrites projecting rostrally in horizontal sections. GC dendrites were covered in gemmules and long-necked spines, up to 10 μm in length (insets - photomicrographs of the regions delineated by the boxes; arrowheads mark cell somata). MTC dendrites, by contrast, were aspiny. In the MTC shown (Bi) an apical dendrite can be followed rostrally, where it ramifies in an olfactory glomerulus (inset). Aii and Bii, traces of spontaneous synaptic activity from the cells in Ai and Bi show the characteristic differences between GC and MTC electrophysiology at rest. In the nose-brain preparation, large (> 5 mV) spontaneous depolarizing potentials in GCs occurred at rates of ≈5 Hz, while spontaneous synaptic activity was less prominent in MTCs. Rinput, input resistance. Aiii and Biii, lateral olfactory tract (LOT) stimulation evoked an antidromic AP in MTCs, which, in turn, synaptically activated the GC population. The antidromic AP in MTCs rose straight from baseline. Hyperpolarization (lower trace) did not block the AP but reversed the synaptically mediated after-hyperpolarization (the AP is truncated in the lower trace). In GCs, LOT stimulation produced an EPSP that increased at more hyperpolarized levels, while the AP was blocked.

    Journal:

    Article Title: Contribution of a calcium-activated non-specific conductance to NMDA receptor-mediated synaptic potentials in granule cells of the frog olfactory bulb

    doi: 10.1113/jphysiol.2002.024638

    Figure Lengend Snippet: OB neurones were distinguished by their anatomical and electrophysiological properties in blind whole-cell recordings. Ai and Bi, reconstructions of neurobiotin-labelled cells from horizontal OB sections (300 μm). GC interneurones (Ai) were characterized by widely branching dendrites projecting rostrally in horizontal sections. GC dendrites were covered in gemmules and long-necked spines, up to 10 μm in length (insets - photomicrographs of the regions delineated by the boxes; arrowheads mark cell somata). MTC dendrites, by contrast, were aspiny. In the MTC shown (Bi) an apical dendrite can be followed rostrally, where it ramifies in an olfactory glomerulus (inset). Aii and Bii, traces of spontaneous synaptic activity from the cells in Ai and Bi show the characteristic differences between GC and MTC electrophysiology at rest. In the nose-brain preparation, large (> 5 mV) spontaneous depolarizing potentials in GCs occurred at rates of ≈5 Hz, while spontaneous synaptic activity was less prominent in MTCs. Rinput, input resistance. Aiii and Biii, lateral olfactory tract (LOT) stimulation evoked an antidromic AP in MTCs, which, in turn, synaptically activated the GC population. The antidromic AP in MTCs rose straight from baseline. Hyperpolarization (lower trace) did not block the AP but reversed the synaptically mediated after-hyperpolarization (the AP is truncated in the lower trace). In GCs, LOT stimulation produced an EPSP that increased at more hyperpolarized levels, while the AP was blocked.

    Article Snippet: Neuronal labelling Neurobiotin (Vector Laboratories, Burlingame, CA, USA) was added to the intracellular pipette solution (0.5 % w/v) and cells were filled by passive diffusion.

    Techniques: Activity Assay, Blocking Assay, Produced